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Spiking/recovery tests were performed to determine the optimal dilution factor, particularly for the kits lacking manufacturer-provided serum dilution guidelines [human pro-BDNF DuoSet ELISA kit (#DY3175, R&D Systems) and high-sensitivity pro-BDNF human ELISA kit (#SK00752-09, Aviscera Bioscience)]

Eur Thyroid J (2019) 8(6):30711

[38:29] That's where I've started pushing back against the convention being established in the holistic and functional medicine community where everyone is enamored by testing

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However, keep in mind supplements have side effects that may impact your weight in other ways, like affecting your sleep or mood